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. 2020 Jul 22;13:7111–7123. doi: 10.2147/OTT.S243542

Figure 2.

Figure 2

DUXAP8 knockdown inhibited the viability and migration of NSCLC cells. (A) DUXAP8 expression in BEAS-2B, H1299, A549, H460 and PC-9 cells was detected by RT-qPCR (n=3). (B) RT-qPCR was used to measure the expression of DUXAP8 in A549 and H1299 cells transfected with siRNAs against DUXAP8 or si-NC (n=3). (C and D) CCK-8 assay was used to determine the viability of NSCLC cells transfected with si-DUXAP8 or si-NC (n=3). (E) The number of colonies of NSCLC cells transfected with si-DUXAP8 or si-NC was detected by cell colony formation assay (n=3). (F) The migratory ability of NSCLC cells transfected with si-DUXAP8 or si-NC was examined by transwell migration assay (n=3). (G and H) The protein expression of c-myc, Cyclin D1, MMP9 and E-cad in NSCLC cells transfected with si-DUXAP8 or si-NC was detected by Western blot (n=3).