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. 2020 Jul 22;13:7111–7123. doi: 10.2147/OTT.S243542

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© 2020 Yin et al.

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DUXAP8 downregulation dampened the glycolysis in NSCLC cells. (A–C) Glucose uptake (A), lactate release (B), and ATP production level (C) were measured in A549 and H1299 cells transfected with si-DUXAP8 or si-NC by Glucose Uptake Colorimetric Assay Kit, Lactate Assay Kit II, and ATP Colorimetric Assay Kit, respectively (n=3). (D–G) The quantification of OCR (D and E) and ECAR (F and G) in NSCLC cells transfected with si-DUXAP8 or si-NC was measured by Seahorse Extracellular Flux Analyzer XF96 assays (n=3). (H and I) The protein expression of HK2 and LDHA in NSCLC cells transfected with si-DUXAP8 or si-NC was examined by Western blot analysis (n=3).