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. 2020 Jul 22;3(9):e201900619. doi: 10.26508/lsa.201900619

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© 2020 Kim et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 7. — (A) Relative mitochondrial membrane potentials. (B, C) Cellular oxygen consumption rates (B) and relative ATP levels (C) in indicated cell types. (D) Immunoblot analyses of p-CREB and p-AMPK-α in shCon, shSpr, and BH4 (20 μM)-treated shSpr HL-1 cells. (E) Representative Western blot of siCon and Camkk2-knockdown (siCaMKK2-1 or siCaMKK2-2) HL-1 cells in the presence or absence of BH4 (20 μM). (F) The proposed mechanism of BH4–CaMKK2-associated heart and mitochondrial regulation. *P < 0.05 versus shCon; ^#P < 0.05 versus shSpr (n = 3/group).

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