Skip to main content
. 2020 Jul 22;3(9):e201900619. doi: 10.26508/lsa.201900619

Figure S3. BH4 suppresses proteasomal activity and fibrosis in Otsuka Long–Evans Tokushima Fatty rats.

Figure S3.

(A) Zymographic analysis comparing matrix metalloproteinase (MMP) activity in the hearts of experimental animals. (B) Relative levels of MMP-2, ProMMP-2, and ProMMP-9. (C) 26S proteasome activity in cardiac tissues from model animals. Lactacystin, a 26S proteasome inhibitor, was used as a positive control. (D) Immunoblot images of selected pro-fibrotic proteins. (E) Relative protein levels of inactive TGF-β1, active TGF-β1, fibronectin, collagen I, collagen III, and monocyte chemoattractant protein-1 normalized against β-tubulin level. TGF-β1, monocyte chemoattractant protein-1, and collagen I and III bands were cropped from the same gel, and fibronectin and β-actin bands were cropped from another gel. Both gels ran simultaneously with 20 μg of each protein (n = 3/group). (F) Immunoblot images and relative protein level of total and phopho-eNOS proteins. (n = 3/group) Data represent the mean ± SEM. *P < 0.05 versus Long–Evans Tokushima Otsuka; #P < 0.05 versus Otsuka Long–Evans Tokushima Fatty.