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. 2020 Jul 22;3(9):e201900619. doi: 10.26508/lsa.201900619

Figure S7. BH4 treatment improves mitochondrial OXPHOS and biogenesis.

Figure S7.

(A) Mitochondrial oxygen consumption rate in isolated WT mouse cardiac mitochondria in the presence or absence of BH4 (20 μM). (B) The mitochondrial mass changes in HL-1 cell in the presence or absence of BH4 (20 μM for 24 or 48 h). The mitochondrial mass was measured by acridine orange 10-nonyl bromide (NAO; Invitrogen). (C) Immunoblot images of selected mitochondrial biogenesis-regulating proteins in the heart of WT or BH4 treated mouse (20 mg/kg/day via i.p. injection for 2 wk).