Figure 5.

Effects of K_V6.4-Met419 on K_V2.1 Heterotetramerization

(A) Wild-Type K_V6.4 co-immunoprecipitates with K_V2.1 when co-expressed in HEK293 cells (pulling down with K_V2.1 or HA-tagged K_V6.4). K_V6.4-Met419 disrupts binding to K_V2.1, and there is significantly reduced binding of HA-tagged K_V6.4 to K_V2.1 when co-expressed with untagged K_V6.4-Met419.

(B) K_V6.4 traffics to the plasma membrane less efficiently when co-expressed with untagged K_V6.4-Met419, indicating a dominant-negative effect. Scale bars indicate 10 μm.

(C) Quantification of K_V6.4 membrane localization by Pearson’s coefficient, assessing co-localization of HA and the Na⁺/K⁺ ATPase membrane marker. Data are from three independent experiments. Error bars indicate SEM, ***p < 0.001