REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-HA mouse monoclonal	Biolegend	Cat# 12B12, #MMS-101P; RRID: AB_2801249
Anti-KV2.1 rabbit polyclonal	Alomone	Cat# APC-012; RRID: AB_2040162
Anit-KV2.1 mouse monoclonal	Abcam	Cat# ab192761; RRID: AB_2750677
Anti-HA rabbit monoclonal	Cell Signaling	Cat# C29F4 #3724: RRID: AB_10693385
Anti-Na+/K+ ATPase rabbit monoclonal	Abcam	Cat# Ab76020; RRID: AB_1310695
Anti β-actin mouse monoclonal	Sigma Aldrich	Cat # A2228; RRID: AB_476697
Anti-mCherry rat monoclonal	ThermoFisher	Cat # M11217; RRID: AB_2536611
Chemicals, Peptides, and Recombinant Proteins
Trypsin	Sigma-Aldrich	T4799
Collagenase	Sigma-Aldrich	C5138
Bovine serum albumin	Sigma-Aldrich	B2064
Fast Blue	Polysciences	17740
Trypsin from Bovine Pancreas	Sigma-Aldrich	T9935
Collagenase, Type 1A	Sigma-Aldrich	C9891
Capsaicin	Sigma-Aldrich	M2028
Stromatoxin-1	Alomone	STS-350
FuGENE HD transfection reagent	Promega	E2311
Critical Commercial Assays
CellsDirect One-Step qRT-PCR kit	Invitrogen	11753100
Amaxa Mouse Neuron Nucleofactor kit	Lonza	VPG-1001
Dynabeads Co-Immuniprecipitation Kit	ThermoFisher	14321D
Kcng4 Taqman primer-probe	Applied Biosciences	Mm01240890_m1
Kcnb1 Taqman primer-probe	Applied Biosciences	Mm00492791_m1
Trpv1 Taqman primer-probe	Applied Biosciences	Mm01246300_m1
Scn10a Taqman primer-probe	Applied Biosciences	Mm00501467_m1
Gapdh Taqman primer-probe	Applied Biosciences	Mm99999915_g1
Experimental Models: Cell Lines
HeLa	Sigma Aldrich	93021013
HEK293	Sigma Aldrich	85120602
Experimental Models: Organisms/Strains
C57BL/6J mice	Envigo	Wild-type
Recombinant DNA
KCNG4 cDNA clone in polioIRESmCherry w/wo HA tag	This manuscript	N/A
Kv2.1 in pCAGGS-IRES2-nucEGFP	Gift from Prof Saitsu	Saitsu et al., 2015
KCNG4.Met419 cDNA clone in polioIRESmCherry w/wo HA tag	This manuscript	N/A
Software and Algorithms
R Studio Version 1.2.5036 for Mac	R	https://rstudio.com/products/rstudio/
pClamp (v10.3)	Molecular Devices	pClamp (v10.3)
Prism (v8)	GraphPad	Prism (v8)
Patchmaster	HEKA	heka.com
Fitmaster v2x90.4	HEKA	heka.com
Igor pro v6.37	WaveMetrics	wavemetrics.com
Patcher’s Power Tools	Max-Planck-Institut	https://www3.mpibpc.mpg.de/groups/neher/index.php?page=aboutppt
Step One version 2.3	Applied Biosystems	N/A
Other
Poly-D-lysine and laminin coated coverslips	BD Biosciences	354087
Nucleofector IIb	Lonza	AAB-1001
Multiclamp 700A amplifier	Molecular Devices	N/A
Digidata 1440A	Molecular Devices	N/A
Rapid change perfusion system	Intracel	EVH-9
EPC-10 amplifier	HEKA	N/A
Pipette puller	Sutter Instruments	P-97
Lebovitz L015 Glutamax	Thermo Fisher Scientific	31415029
Poly-D-lysine coated coverslips	BD Biosciences	354086
SUPERase-inhibitor	Ambion	AM2696