The human SIGLEC14/5 gene polymorphism influences inflammasome activation in response to GBS. Human MDM of the indicated genotypes were infected with GBS WT or the GBS ΔcylE mutant at MOI = 10 bacteria/cell with measurement of IL-1β (a, d) and IL-6 (b, e) release at 16 h by ELISA. The ratio of IL-1β to IL-6 was calculated (c, f). Data are from 5 to 7 different donors per SIGLEC-14/15 genotype. The lines indicate the means ± SEM of each group. ** p < 0.005. WT, wild-type; IL-1β, interleukin-1β; GBS, group B Streptococcus