Table 1.
Sequences for real-time PCR primers.
| Gene | GenBank ID | Sequence (5′⟶3′, forward primer/reverse primer) | Product length (bp) |
|---|---|---|---|
| Nrf2 | XM_013984303.2 | ATCCAGCGGATTGCTCGTAG | 155 |
| TCAAATCCATGTCCTTGGCG | |||
| Keap1 | NM_001114671.1 | TCTGCTTAGTCATGGTGACCT | 158 |
| GGGGTTCCAGATGACAAGGG | |||
| HO1 | NM_001004027.1 | TGATGGCGTCCTTGTACCAC | 71 |
| GACCGGGTTCTCCTTGTTGT | |||
| SOD1 | NM_001190422.1 | CATTCCATCATTGGCCGCAC | 118 |
| TTACACCACAGGCCAAACGA | |||
| GPX1 | NM_214201.1 | CCTCAAGTACGTCCGACCAG | 85 |
| GTGAGCATTTGCGCCATTCA | |||
| GCLC | XM_003482164.4 | CTAGTGGGTAGGCGGACTGG | 81 |
| CGGTGTCGTGCTCTAGCTTC | |||
| GCLM | XM_001926378.4 | GGACAAAACCCAGTTGGAGC | 86 |
| TCACACAGCAAGAGGCAAGA | |||
| GR | AY368271.1 | GTGAGCCGACTGAACACCAT | 141 |
| CAGGATGTGAGGAGCTGTGT | |||
| SREBP1c | NM_214157.1 | GCGACGGTGCCTCTGGTAGT | 218 |
| CGCAAGACGGCGGATTTA | |||
| PPARα | NM_001044526.1 | GGCACTGAACATCGAATGTAGAAT | 80 |
| TGCAACCTTCACAGGCATGA | |||
| ACC | NM_001114269.1 | ATCCCTCCTTGCCTCTCCTA | 208 |
| ACTTCCCGTTCAGATTTCCG | |||
| FAS | NM_001099930.1 | TACCTTGTGGATCACTGCATAGA | 113 |
| GGCGTCTCCTCCAAGTTCTG | |||
| SCD1 | NM_213781.1 | ATTGGGAGCTGTGGGTGAG | 90 |
| AAGTTGATGTGCCAGCGGTA | |||
| HSL | NM_214315.3 | GCAGCATCTTCTTCCGCACA | 195 |
| AGCCCTTGCGTAGAGTGACA | |||
| CPT1α | NM_001129805.1 | TCAAAAACGGCAAGATGGGC | 155 |
| TGGAATGTTGGGGTTGGTGT | |||
| LPL | NM_214286.1 | CACATTCACCAGAGGGTC | 177 |
| TCATGGGAGCACTTCACG | |||
| L-FABP1 | NM_001004046.2 | AGGGGACATCGGAAATCGTG | 103 |
| TCACACTCCTCTCCCAAGGT | |||
| CD36 | NM_001044622.1 | TGACCCAGCACTTGAAGCAA | 130 |
| AAGATATCAGTTAGGAGTCCGATGA | |||
| FATP1 | NM_001083931.1 | AGGTCTGGCGTGGGTCAAAG | 208 |
| GGAGTAGAGGGCAAAGCAGG | |||
| MTTP | NM_214185.1 | AGCAAAATGGTCCGTCGAGT | 114 |
| CGAATGGGGACCACGTTCTA | |||
| MCP1 | NM_214214.1 | AAACGGAGACTTGGGCACAT | 74 |
| GCAAGGACCCTTCCGTCATC | |||
| F4/80 | XM_021083974.1 | TCCTTCTCTTTTGGGGGTGT | 73 |
| GCCATTGACTCCAACGGAGA | |||
| CD11c | XR_002342355.1 | GGAGCAAATGGACAGACCGT | 95 |
| GAATGCAGGTGCAAAGGCAA | |||
| TLR4 | GQ304754 | TTTCTTGCAGTGGGTCGAGG | 161 |
| GGAAGGTGAGAACTGACGCA | |||
| MyD88 | NM001099923.1 | GTGCCGTCGGATGGTAGTG | 65 |
| TCTGGAAGTCACATTCCTTGCTT | |||
| TRAF6 | NM_001105286.1 | GCTGCATCTATGGCATTTGAAG | 71 |
| CCACAGATAACATTTGCCAAAGG | |||
| NF-KB, p65 | NM_001114281.1 | GGGGCGATGAGATCTTCCTG | 110 |
| CACGTCGGCTTGTGAAAAGG | |||
| TNF-α | NM_214022.1 | GCCCTTCCACCAACGTTTTC | 97 |
| CAAGGGCTCTTGATGGCAGA | |||
| IL6 | NM_214399.1 | ACAAAGCCACCACCCCTAAC | 185 |
| CGTGGACGGCATCAATCTCA | |||
| IL1β | NM_214055.1 | ATTCAGGGACCCTACCCTCTC | 92 |
| ATCACTTCCTTGGCGGGTTC | |||
| IL10 | NM_214041.1 | CGGCCCAGTGAAGAGTTTCT | 98 |
| GGCAACCCAGGTAACCCTTA | |||
| β-Actin | XM_003124280.5 | CTCCAGAGCGCAAGTACTCC | 153 |
| AATGCAACTAACAGTCCGCC |
Nrf2: nuclear factor erythroid-derived 2-like 2; Keap1: Kelch-like ECH-associated protein 1; HO1: heme oxygenase 1; SOD1: superoxide dismutase 1; GPX1: glutathione peroxidase 1; GCLC: glutamate-cysteine ligase catalytic subunit; GCLM: glutamate-cysteine ligase modifier subunit; GR: glutathione reductase; SREBP1c: sterol regulatory element-binding protein 1c; FAS: fatty acid synthase; ACC: acetyl-CoA carboxylase; SCD1: stearoyl-CoA desaturase 1; PPARα, peroxisome proliferator-activated receptor alpha; HSL: hormone-sensitive lipase; CPT1α: carnitine palmitoyltransferase 1 alpha; LPL: lipoprotein lipase; L-FABP1: liver fatty acid-binding proteins 1; CD36: cluster of differentiation 36; FATP1: fatty acid transport proteins 1; MTTP: microsomal triglyceride transfer protein; MCP1: monocyte chemotactic protein 1; F4/80: adhesion G protein-coupled receptor E1; CD11c: integrin alpha X; TLR4: toll-like receptor 4; MyD88: myeloid differentiation factor 88; TRAF6: tumor necrosis factor receptor-associated factor 6; NF-κB: nuclear factor-κB; TNF-α: tumor necrosis factor alpha; IL-6: interleukin 6; IL1β: interleukin 1β; IL10: interleukin 10; β-actin: beta actin.