Fig. 2.

Increased conversion of LC3-I to LC3-II and downregulated ratio of p62 to β-actin caused by PCV3 capsid protein

Cells were first transfected with pCMV-capsid (Cap) or the control plasmid pCMV. Rapamycin (Rapa) was used as positive control. After 36 h of transfection, the whole cell lysates were subjected to western blotting for β-actin, LC3, p62, and PCV3 Cap. (a) A representative blot showing the effects of PCV3 Cap on target proteins. (b, c) Ratios of LC3-II to LC3-I and p62 to β-actin normalized to mock transfection set at 1.0. Data were expressed as mean±standard error of the mean (SEM), n=3. ^* P<0.05; ^** P<0.01