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. 2020 May 14;295(30):10125–10135. doi: 10.1074/jbc.RA120.013654

Figure 3.

Figure 3.

Structure-guided disruption of S. cerevisiae Tip20•Sec20 complex formation. A, size-exclusion chromatography demonstrates robust binding (blue) between WT S. cerevisiae Tip20 (green) and MBP-Sec20NTD (red). B, substitution of Sec20 Leu-149 with aspartate has little effect on complex formation. C, substitution of Sec20 Val-82 with aspartate partially compromises binding to Tip20. D, the two substitutions in combination have a stronger effect on binding. The data presented for Tip20 alone in B–D are identical to those in A.