Skip to main content
. 2020 Jun 3;295(30):10212–10223. doi: 10.1074/jbc.RA119.012263

Figure 3.

Figure 3.

miR-223-3p KO impairs skeletal muscle regeneration after injury. A–B, RT-PCR assessment of Myod1 and Myog expression in the muscles of WT and miR-223-3p KO mice on 0 day and 3 days after CTX injury (n = 4 per group). C, representative eMHC immunofluorescence (green) of muscles of WT and miR-223-3p KO mice at 7 days after CTX injury. The nuclei were counterstained with DAPI (blue). Scale bar, 50 μm. D, percentages of eMHC-positive areas in fields of view from muscles of WT and miR-223-3p KO mice at 7 days after CTX injury (n = 3 per group). E, representative HE staining of muscles of WT and miR-223-3p KO mice at 0 day, 14 days, and 30 days after CTX injury. Scale bar, 50 μm. F, representative WGA staining (green) of muscles of WT and miR-223-3p KO mice at 0 day, 14 days, and 30 days after CTX injury. The nuclei were counterstained with DAPI (blue). Scale bar, 50 μm. G, mean myofiber CSA of muscles of WT and miR-223-3p KO mice at 0 day, 14 days, and 30 days after CTX injury (n = 3–4 per group; for each sample, ≥300 myofibers were measured). H, CSA distributions of muscles of WT and miR-223-3p KO mice at 30 days after CTX injury. Data are expressed as the mean ± S.D. *, p < 0.05; **, p < 0.01 by unpaired two-tailed Student's t test.