miR-223-3p KO increases the inflammatory response in injured skeletal muscle.
A, representative flow cytometry analysis of CD45+ leukocytes, CD45+ CD11b+ monocytes, and CD45+ CD11b+ F4/80+ macrophages in the muscles of WT and miR-223-3p KO mice at 1 day and 3 days after CTX injury. B–D, percentages of CD45+, CD45+ CD11b+, and CD45+ CD11b+ F4/80+ cells in the muscles of WT and miR-223-3p KO mice at 1 day and 3 days after CTX injury (n = 3–4 per group). E–F, RT-PCR assessment of Ptprc and Itgam expression in the muscles of WT and miR-223-3p KO mice at 1 day and 3 days after CTX injury (n = 3–4 per group). Data are expressed as the mean ± S.D. *, p < 0.05; **, p < 0.01 by unpaired two-tailed Student's t test.