Figure 5.

miR-223-3p KO increases the inflammatory response in injured skeletal muscle. A, representative flow cytometry analysis of CD45⁺ leukocytes, CD45⁺ CD11b⁺ monocytes, and CD45⁺ CD11b⁺ F4/80⁺ macrophages in the muscles of WT and miR-223-3p KO mice at 1 day and 3 days after CTX injury. B–D, percentages of CD45⁺, CD45⁺ CD11b⁺, and CD45⁺ CD11b⁺ F4/80⁺ cells in the muscles of WT and miR-223-3p KO mice at 1 day and 3 days after CTX injury (n = 3–4 per group). E–F, RT-PCR assessment of Ptprc and Itgam expression in the muscles of WT and miR-223-3p KO mice at 1 day and 3 days after CTX injury (n = 3–4 per group). Data are expressed as the mean ± S.D. *, p < 0.05; **, p < 0.01 by unpaired two-tailed Student's t test.