Figure 5.

Effects of cyclic nucleotide analog inhibitors on RQ PKG1 activity. In vitro basal kinase activity of purified R177Q PKG1α or R192Q PKG1β measured in the presence of increasing concentrations of Rp-8-pCPT–cGMPS (A and B) or Rp-8-pCPT-PET–cGMPS (C and D). E, In vitro kinase activity of isolated PKG1 catalytic domain (CD) measured in the presence of increasing Rp-8-pCPT-PET-cGMPS concentrations. For all reactions, basal activity in the absence of inhibitor was set at 100%. Data are the average from three experiments using three independent protein preparations. Bars show means ± S.D., n = 3.