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. 2020 Jun 10;295(30):10456–10467. doi: 10.1074/jbc.RA120.014081

Figure 4.

Figure 4.

Relative phuR mRNA and protein levels for the hasR allelic strains under iron-depleted conditions. A, mRNA isolated at 0, 2, 4, and 6 h following growth in M9 minimal media. mRNA values represent the means from three biological experiments, each performed in triplicate and normalized to PAO1 WT at the same time point. Error bars represent the standard deviations from three independent experiments performed in triplicate. The indicated p values were normalized to mRNA levels of PAO1 WT at the same time point: *, p < 0.05; **, p < 0.005. B, normalized density (n = 3) was determined on Western blots for three separate biological replicates. The indicated p values were normalized to PAO1 WT at the same time point: *, p < 0.05; **, p < 0.005. C, representative Western blotting of PAO1 WT and the hasR allelic strains. Total protein (5 μg) was loaded in each well. RNApolα was used as a loading control.