Figure 5.

Heme utilization by the hasR allelic strains when supplemented with ¹³C-heme. A, LC-MS/MS analysis of the BVIX isomers supplemented with 1 μm ¹³C-heme at 4 h. Biliverdin values represent the standard deviations from four biological replicates. The indicated p values are for each strain compared to the PAO1 WT for the respective BVIX isomers: *, p < 0.05; **, p < 0.005. B, ICP-MS analysis of intracellular iron levels. The indicated p values are for each strain compared with PAO1 WT: *, p < 0.05; **, p < 0.005.