Figure 9.

Relative hasR and hasAp mRNA levels for the hasR allelic strains under holo-HasAp-supplemented conditions. A, hasR relative mRNA levels. B, hasAp relative mRNA levels. mRNA was isolated at 0, 2, 4, and 6 h following growth in M9 supplemented with 1 μm holo-HasAp. mRNA values represent the means from three biological experiments, each performed in triplicate and normalized to 0 h for each strain. Error bars represent the standard deviations from three independent experiments performed in triplicate. The indicated p values were normalized to mRNA levels of PAO1 WT at the same time point: *, p < 0.05; **, p < 0.005.