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Pyrophosphorolysis of CMP and 2′C Modified CTP analogues. The plots show the pyrophosphorolysis of CMP (31.25, 62.5, 125, 500, and 1000 μm) (A), 2′C-Me-CMP (15.6, 31.3, 62.5, 125, 250, and 500 μm) (B) and 2′C-Me-2′F-CMP (15.6, 31.3, 62.5, 125, 250, and 500 μm) (C). The data were fit using Scheme 2 using KinTek Explorer. Solid lines represent best fit resulting from the fitting process. Results are summarized in Table 2.
