Figure 7.

Primary amino acid sequence alignment of chromosomal Lit and accessory Lit2 proteins. Sequences of the chromosomal Lit (Lit1) and the copper-resistance associated accessory transposon/plasmid located (Lit2) paralogs from the indicated strains was generated by STRAP (36). Highly conserved amino acids are colored (purple for 100% and yellow for retention in all but one sequence). Residues deemed essential (green) or nonessential (blue) by Mut-seq analysis and confirmed for function (Fig. 6D) are indicated. Predicted TM segments (by Protter (26); see Fig. 8) are boxed in gray with uncertainty in the boundaries of loop 3 (L3) denoted by brackets and asterisks (Table S2).