TABLE 5.
Throughput and workflow evaluation for four EUA molecular SARS-CoV-2 assays
| SARS-CoV-2 molecular assay |
Throughput (no. of samples per run) |
Input vol per sample/ elution vol |
HoT (per run) |
Assay run time |
User results interpretation |
Overall TAT |
|---|---|---|---|---|---|---|
| Modified CDC | 24 | 110 μl/110 μl | ∼1.5 h | ∼90 min | Yes | ∼3.0 h |
| DiaSorin Molecular | 8 per disc | 50 μl/NAa | <16 min | ∼90 min | No | ∼1.8 h |
| GenMark | 6 per tower | 200 μl/NA | <12 min | ∼90 min | No | ∼1.7 h |
| Hologic | 120 | 360 μl/50 μl | ∼2.0 h | ∼4 h 35 min | No | ∼6.6 h |
a
NA, not applicable.