TABLE 1.
Initial sensitivity assessment of QIAstat-Dx respiratory 2019-nCoV panel (Qiagen) and the WHO-recommended Charité RT-PCR assay for the E gene and RdRp gene (4) on serial dilutions of a positive nasopharyngeal clinical sample (strain 1)
| Dilution | QIAstat-Dx |
E gene |
RdRp gene |
|||
|---|---|---|---|---|---|---|
| CT value | Interpretation | CT value | Interpretation | CT value | Interpretation | |
| 1/4 | 21.1 | Positive | 21.5 | Positive | 23.0 | Positive |
| 1/40 | 25.7 | Positive | 25.6 | Positive | 26.1 | Positive |
| 1/400 | 28.0 | Positive | 29.6 | Positive | 31.3 | Positive |
| 1/4,000 | 31.0 | Positive | 33.1 | Positive | Not detected | Negative |
| 1/40,000 | 34.8 | Positive | 35.7 | Positive | Not detected | Negative |
| 35.2 | Positive | 36.0 | Positive | Not detected | Negative | |
| 35.3 | Positive | 36.2 | Positive | Not detected | Negative | |
| 1/400,000 | 36.3 | Positive | 37.3 | Positive | Not detected | Negative |
| 37.1 | Positive | 37.6 | Positive | Not detected | Negative | |
| 37.4 | Positive | 38.4 | Positive | Not detected | Negative | |
| Not detected | Negative | 38.5 | Positive | Not detected | Negative | |
| Not detected | Negative | 38.7 | Positive | Not detected | Negative | |
| 1/4,000,000 | 35.9 | Positive | 38.4 | Positive | Not detected | Negative |
| Not detected | Negative | 40.0 | Negative | Not detected | Negative | |
| Not detected | Negative | 40.4 | Negative | Not detected | Negative | |
| Not detected | Negative | Not detected | Negative | Not detected | Negative | |
| Not detected | Negative | Not detected | Negative | Not detected | Negative | |