TABLE 3.
Workflow analysis comparing laboratory-modified CDC COV assay, ACOV assay, and IDNOW assay
| Parametera | CDC COV | ACOV | IDNOW |
|---|---|---|---|
| Off-board lysis | Yes | No | No |
| Specimen processing and set up | 1.75 | 1.00 | 0.03 |
| Instrument extraction time | 1.30 | 4.0 | 0.05 |
| Amplification and real-time detection | 1.25 | 2.25 | 0.22 |
| Manual interpretation and result entry | 0.5 | 0.75 | 0.02 |
| Total time (h) to resultb | 4.8 | 8.0 | 0.27 |
| No. samples processed in 8-h shift per instrument | 58c | 94d | 32e |
a
Times (hours) per batch for the in-house laboratory-developed test (LDT) and Abbott m2000 assays, and per sample for the ID Now assay.
b
Times (hours) from sample processing through result reporting.
c
A maximum of 58 patient samples, not including external positive control, negative control, negative template control, RNase P control.
d
A maximum of 94 patient samples, not including external positive and negative controls.
e
Assumes continuous processing of 32 patient samples, all with negative results. Results for positive samples may be generated within 5 min, and results for negative samples are generated within 13 min.