Table 2.
Biochemical parameters for trypanosomatid PFKs incorporating all previous studies and the values obtained from this work.
| Species | Source | K0.5 ATP (mm) | K0.5 F6P (mm) |
V max µmol·min−1·mg−1 |
Conditions | Reference |
|---|---|---|---|---|---|---|
| T. cruzi | Partially purified parasite | 0.04 | 1.31 (0.37 if 0.5 mm AMP) | 0.4 |
1 mm ATP 1 mm F6P |
11, 18 |
| Recombinant (E. coli) | 0.0125 | – | – | 1 mm F6P | 12, 19 | |
| Purified parasite | 0.025 | 2.8 | 0.07 |
0.5 mm ATP 2 mm F6P |
13, 20 | |
| Whole parasite homogenate | – |
0.29 (epimastigotes) 0.15 (metacyclics) |
40.3 | 1 mm ATP | 14, 21 | |
| Partially purified parasite | 1.9 (0.05 if 3 mm AMP) | 1.75 (0.8 if 0.1 mm AMP) | – | Not stated | 15, 22 | |
| Recombinant (E. coli) | 0.095 | 0.31 | 0.068 |
3 mm F6P 1.5 mm ATP |
This work | |
| T. brucei | Purified parasite | 0.065 | 0.999 | – |
5 mm F6P 1 mm ATP |
16, 23 |
| Recombinant (E. coli) | 0.144 | 1.15 | 0.045 |
0.5 mm ATP 1.6 mm AMP |
17, 24 | |
| Purified parasite | 0.026 | 0.82 | 0.267 | 2 mm ATP | 18, 25 | |
| Recombinant (E. coli) | 0.050 | 0.37 | 0.045 |
3 mm F6P 1.5 mm ATP |
This work | |
| L. mexicana | Whole parasite homogenate | – | – |
0.138 (promastigotes) 0.089 (amastigotes) |
0.4 mm ATP 3.3 mm F6P |
10, 17 |
| L. donovani | Recombinant (E. coli) | – | 3.6 (0.157 if 1.5 mm AMP) | Not stated | 1 mm ATP | 9, 16 |
| Whole parasite homogenate | 0.08 | 10 (0.5 if 3 mm AMP) |
0.29 0.43 with 3 mm AMP |
Not stated | 8, 15 | |
| L. infantum | Recombinant (E. coli) | 0.050 | 2.89 | 0.014 |
3 mm F6P 1.5 mm ATP |
This work |