Table 4.
Steady‐state SPR affinities for key ligands for each trypanosomatid PFK. (N = 3; values are mean averages with standard errors; surface densities: TbPFK = 3200 RU; TcPFK = 3100 RU; LiPFK = 1400 RU).
| TcPFK | TbPFK | LiPFK | |
|---|---|---|---|
| AMP | |||
| Kd AMP (mm) | 1.04 (0.30) | 0.47 (0.02) | 2.3 (0.20) |
| Kd AMP + 1 mm ATP (mm) | 1.69 (0.10) | 1.93 (0.30) | 12.2 (4.80) |
| ADP | |||
| Kd ADP (mm) | 0.73 (0.10) | 0.56 (0.08) | 0.16 (0.02) |
| Kd ADP + 1 mm ATP (mm) | 2.13 (0.32) | 1.51 (0.11) | 0.86 (0.13) |
| ATP | |||
| Kd ATP (µm) | 35.4 (5.6) | 15.8 (2.3) | 16.8 (2.1) |
| Kd ATP + 1 mm AMP (µm) | 36.7 (3.3) | 15.1 (1.7) | 19.3 (1.1) |
| F6P | |||
| Kd F6P (mm) | No binding | No binding | No binding |
| Kd F6P + 1 mm ATP (mm) | Weak binding | Weak binding | Weak binding |