Table 4.
Point‐of‐care identification of Neoparamoeba perurans by LAMP assay
| Gill score range | N. perurans | Atlantic salmon | ||
|---|---|---|---|---|
| Tp | +ve | Tp | +ve | |
| 0.1–1 | 32:50 ± 7:10 | 11/22 | 24:10 ± 6:0 | 21/22 |
| 1.1–2 | 35:30 ± 6:00 | 13/23 | 24:30 ± 5:10 | 23/23 |
| 2.1–3 | 29:00 ± 8:05 | 5/9 | 22:40 ± 4.45 | 9/9 |
| 3.1–3.6 | 29:05 ± 4:20 | 6/6 | 16:45 ± 1:40 | 6/6 |
DNA was extracted from non‐lethal gill swabs using the QuickExtract method. The Atlantic salmon elongation factor 1a LAMP assay was used as an internal control. LAMP detection expressed as time of positivity Tp (mm:ss). The visual gill index expressed as the average of the 16 hemibranch scores. Number of positive samples (+ve) expressed as: number of positive swabs/total number of swabs analysed per gill score range.