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. 2020 Jul 24;11:2041731420943833. doi: 10.1177/2041731420943833

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 1. — Organotypic slice culture rat model of fetal myelomeningocele (MMC) hydrogel patch treatment. (a) Gross inspection of representative lumbosacral defect (dotted oval) in a fetal MMC rat after maternal retinoic acid exposure. (b) H&E sagittal section through fetal MMC rat demonstrating lumbosacral defect (dotted oval, magnification: 4×). (c) Gross appearance of intact rat MMC spinal cords adjacent to an agarose block (asterisk). (d) Schematic view of MMC organotypic system showing spinal cord slices encapsulated within a hydrogel patch. Membrane inserts allow for nutrient absorption to ensure viability. No donor cells are depicted. (e) Representative transverse section (400 µm) of MMC lumbar spinal cord embedded in fibrin hydrogel on brightfield microscopy (day 0, magnification: 4×). Note the preservation of gross topography including median fissures. (f) Representative longitudinal section (400 µm) of MMC lumbar spinal cord embedded in fibrin hydrogel (day 0, magnification: 4×), white arrow = caudal end.