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. 2020 Jul 24;11:2041731420943833. doi: 10.1177/2041731420943833

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 5. — Baseline characteristics of cytoarchitecture and neural cell phenotypes within the myelomeningocele (MMC) lumbar spinal cord and slice cultures at E21. (a) Representative native MMC lumbar spinal (transverse sections) using TuJ1, NeuN, MAP2, and GFAP antibodies demonstrate a reduction in dorsal elements and predominance of neuronal phenotypes (magnification: 4×). (b) Quantification of neural phenotypes within transverse sections of fetal MMC organotypic slice cultures (OSCs) based on optical density (y-axis) calculated from immunohistochemistry data (n = 5/group). Values are presented as mean ± SEM, n = 4 independent biological replicates. (c) Quantitative gene expression showing robust neurotrophic activity of fetal MMC OSCs at 24 h based on brain-derived neurotrophic factor (Bdnf) and neurotrophin-4/5 (Ntf4) upregulation. Age-matched rat dorsal root ganglion (DRG) RNA was used as a positive control. Values are presented as mean ± SEM, *p ⩽ 0.01 (Mann–Whitney), n = 5 independent biological replicates.