Figure 6.

T3SS‐incompetent infection elicits an antimicrobial from PMVECs. A, Whereas infection with T3SS‐incompetent ΔPcrV produces amyloids that are not cytotoxic, they are distinctly and consistently antibacterial in a time‐dependent manner. n ≥ 7 (7‐34) with each independent experiment conducted in triplicate, repeated measures two‐way ANOVA with Bonferroni post hoc; mean ± SEM; *P < .05, **P < .01, ***P < .001. B, T3SS‐incompetent infection elicited endothelial antimicrobial production requires interaction between the bacterium and the host cell. Moreover, active injection of ExoY into host cells is required for the ablation of antimicrobial activity secondary to ExoY⁺ infection. n ≥ 3 with three technical replicates per each independent experiment, two‐way ANOVA with Bonferroni post hoc; mean ± SEM; *P < .05, **P < .01, ***P < .001. C, Boiling cell supernatants from ΔPcrV infection for 30 minutes followed by submersion in an ice bath does not diminish antimicrobial efficacy. n ≥ 4 with each independent experiment conducted in triplicate, repeated measures two‐way ANOVA with Bonferroni post hoc; mean ± SEM; *P < .05, **P < .01, ***P < .001. D, The potential of the endothelial antimicrobial to induce bacterial killing was ascertained by incubating supernatants with bacteria followed by serial dilution and plating experiments. n ≥ 4 with three technical replicates per biological replicate, one‐way ANOVA with Dunnet's multiple comparison post hoc; mean ± SEM; *P < .05