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. 2020 Jul 24;40(7):BSR20200825. doi: 10.1042/BSR20200825

Figure 3. Identification of the potential miRNA sponged by ciRS-7.

Figure 3

(A) Schematic representation of the predicted potential binding sites of miR-641 with ciRS-7 (http://starbase.sysu.edu.cn), and the mutation sites for specific assay. (B) Double luciferase reporter assay in the SKOV3 and A2780 cells co-transfected with ciRS-7 WT or mutated reporter and miR-641 mimics. (C) The miR-641 expression level was down-regulated in the human OC cells (SKOV3, A2780, OV2008, IGROV1 and ES-2) than in the normal human cervical epithelial HOSE cells tested by qRT-PCR. (D) The miR-641 expression levels in paired OC (n=40) and adjacent normal (n=40) tissues from 40 OC patients (same samples as in Figure 1A) detected by qRT-PCR. (E) The miR-641 expression levels in ciRS-7 silenced SKOV3 and A2780 cells detected by qRT-PCR. (F) Pearson correlation analysis of the association between miR-641 with ciRS-7 in the OC tissues from 40 OC patients (same samples as in Figure 1A). Relative miR-641 expression was calculated by 2−ΔΔCt method using U6 as the internal control. All the experiments were repeated for three times; *P<0.01; **P<0.01.