Figure 2.

ICG‐001 increases the epithelial barrier function in PAECs. PAECs were obtained from human bronchial tissue of normal lung transplant donors (n = 6). Cells were seeded in duplicates at a density of 5 × 10⁴ cells/well in 8‐well electrode arrays connected to ECIS system, grown to confluence for 3 days, growth factor/hormone deprived overnight, and pretreated with 10 µM of ICG‐001 or vehicle control for 3 h. (A) Resistance and (B) capacitance values were normalized to the time point of addition of ICG‐001. After 3 h, the cells were wounding by electroporation. (C) Resistance and (D) capacitance values were normalized to the lowest and highest points respectively after wounding. Data are presented as mean ± SEM, and two‐way ANOVA was used to determine the statistical significance; *P < .05