Figure 3.

The end product of CB CD34+ derived from donor I, II, and III‐ in vitro differentiation into red blood cells. (a) Exemplary image of the end product (input sample) for cells derived from donor I. Scale bars correspond to 20 µm. (b) Size and deformability. (c) Equal probability contour plots (the same number of cells fall between each pair of contour lines) of deformation vs cell size (expressed as projected cell area in µm²) for enucleated (pink) and nucleated (purple) cells and nuclei (gray). (d) Histograms of FSC‐A parameter reflecting relative sizes of enucleated and nucleated cells as well as the free‐floating nuclei, measured by flow cytometry. The number of events on each diagram is around 10,000—split accordingly between each subpopulation [Color figure can be viewed at wileyonlinelibrary.com]