Table 1.
Changing cell culture medium composition for the 21 days CB CD34+ differentiation protocol
| Day | Medium composition |
|---|---|
| 0–8 | 60 ng/ml recombinant human stem cell factor (SCF) (cat. 300‐07; PeproTech) |
| 5 ng/ml recombinant human IL‐3 (cat. 200‐03; PeproTech) | |
| 3 U/ml erythropoietin (EPO) (clinical grade material; Roche) | |
| 1 µM hydrocortisone (cat. H0888; Sigma‐Aldrich) | |
| 8–14 | 10 ng/ml SCF |
| 3 U/ml erythropoietin | |
| 1 µM hydrocortisone | |
| 300 µg/ml holotransferrin | |
| 14–21 | 3 U/ml erythropoietin |
| 300 µg/ml holotransferrin |