Figure 2.

Bio‐orthogonal fluorescent labeling of BCNK‐modified IL‐12α is fast and efficient. A)–C) Cells expressing IL‐12α D31* constructs at 0.25 mm BCNK were treated with the indicated concentrations of SiR‐tetrazine fluorophore for 15 min and analyzed by A) in‐gel fluorescence of lysates (IL‐12α D31BCNK is marked with a red arrowhead), B) after immunoprecipitation (IP), and C) by immunoblotting (IB). The + and − lanes refer to the presence or absence of BCNK. SiR‐tetrazine fluorophore was present in all samples. D) Left: Representative gel for a reaction time course between 400 nm SiR‐tetrazine and IL‐12α D31BCNK analyzed by in‐gel fluorescence. Right: Cell lysates were additionally analyzed by immunoblotting.