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. Author manuscript; available in PMC: 2020 Jul 27.
Published in final edited form as: Cell Rep. 2020 Jun 23;31(12):107784. doi: 10.1016/j.celrep.2020.107784

Figure 4. Fetal Antigen Stimulation during Secondary Pregnancy Overrides Cytolysis and Re-expansion of Maternal Memory CD8+ T Cells.

Figure 4.

(A) Schematic showing the transfer of splenocytes from Act-OVA mice on the C57BL/6 (H-2b) background into each group of mice, including PP21 mice after allogeneic pregnancy sired by Act-OVA male mice on the BALB/c background, and pregnant mice mid-gestation during secondary pregnancy sired by Act-OVA or non-transgenic male mice on the BALB/c background.

(B) Number of H-2Kb:OVA257–264 CD8+ T cells and 7 days following intravenous transfer of B6 (H-2b) Act-OVA (OVA+) splenocytes for each group of mice described in (A).

(C) Absolute number (left) or normalized ratio (right) of Act-OVA (OVA+) splenocytes recovered from the spleen and pooled peripheral lymph nodes 7 days following adoptive transfer for each group of mice described in (A).

Data are from at least 3 independent experiments, each with similar results, with each point representing data from an individual mouse.

Bar, mean ± SEM. *p < 0.05, **p < 0.01, and ****p < 0.001.