| Antibodies |
| PE/Cy5 anti-mouse/human CD11b Antibody, Clone M1/70 |
BioLegend |
Cat# 101210; RRID: AB_312793 |
| PE/Cy5 anti-mouse/human CD45R/B220 Antibody, Clone RA3-B62 |
BioLegend |
Cat# 103210; RRID: AB_312995 |
| PE/Cy7 anti-mouse CD62L Antibody, Clone MEL-14 |
BioLegend |
Cat# 104418; RRID: AB_313103 |
| Brilliant Violet 605 anti-mouse Antibody, Clone 29F.1A12 |
BioLegend |
Cat# 135220; RRID: AB_2562616 |
| APC anti-mouse CD152 (CTLA-4) Antibody, Clone UC10–4B9 |
BioLegend |
Cat# 106310; RRID: AB_2087653 |
| Alexa Fluor 700 anti-mouse/human CD44 Antibody, Clone IM7 |
BioLegend |
Cat# 103026; RRID: AB_493713 |
| Brilliant Violet 650 anti-mouse IFN-gamma Antibody, Clone XMG1.2 |
BioLegend |
Cat# 505832; RRID: AB_2734492 |
| APC anti-mouse TNF-alpha Antibody, Clone MP6-XT22 |
BioLegend |
Cat# 506308; RRID: AB_315429 |
| Brilliant Violet 421 anti-mouse IL-2 Antibody, Clone JES6–5H4 |
Biolegend |
Cat# 503826; RRID: AB_2650897 |
| PE/Cy7 anti-mouse CD45.1 Antibody, Clone A20 |
BioLegend |
Cat# 110730; RRID: AB_1134168 |
| Brilliant Violet 421 anti-mouse CD45.2 Antibody, Clone 104 |
BioLegend |
Cat# 109832; RRID: AB_2565511 |
| eFluor 450 anti-mouse CD4 Antibody, Clone GK1.5 |
Thermo Fisher Scientific |
Cat# 48–0041-82; RRID: AB_10718983 |
| APC-eFluor 780 anti-mouse CD8alpha Antibody, Clone 53–6.7 |
Thermo Fisher Scientific |
Cat# 7–0081-82; RRID: AB_1272185 |
| PE/Cy5 anti-mouse CD11c Antibody, Clone N418 |
Thermo Fisher Scientific |
Cat# 15–0114-82; RRID: AB_468717 |
| PE/Cy5 anti-mouse F4/80 Antibody, Clone BM8 |
Thermo Fisher Scientific |
Cat# 15–4801-82; RRID: AB_468798 |
| PE/Cy7 anti-mouse CD197 (CCR7) Antibody, Clone 4B12 |
Thermo Fisher Scientific |
Cat# 25–1971-82; RRID: AB_469652 |
| FITC anti-mouse CD223 (LAG-3) Antibody, Clone C9B7W |
Thermo Fisher Scientific |
Cat# 11–2231-82; RRID: AB_2572484 |
| eFluor 450 anti-mouse CD366 (TIM3) Antibody, Clone 8B.2C12 |
Thermo Fisher Scientific |
Cat# 48–5871-82; RRID: AB_2574081 |
| FITC anti-mouse CD4 Antibody, Clone GK1.5 |
Thermo Fisher Scientific |
Cat# 11–0041-85; RRID: AB_464893 |
| PE/Cy7 anti-mouse CD8alpha Antibody, Clone 53–6.7 |
Thermo Fisher Scientific |
Cat# 25–0081-82; RRID: AB_469584 |
| PE/Cy7 anti-mouse CD3epsilon Antibody, Clone 145–2C11 |
Thermo Fisher Scientific |
Cat# 25–0031-82; RRID: AB_469572 |
| InVivoPlus anti-mouse CD4 Antibody Clone GK1.5 |
BioXCell |
Cat# BP0003–1; RRID: AB_1107636 |
| InVivoPlus anti-mouse CD8alpha Antibody, Clone 2.43 |
BioXCell |
Cat# BP0061; RRID: AB_1125541 |
| InVivoPlus anti-mouse PD-L1 Antibody, Clone 10F.9G2 |
BioXCell |
Cat# BP0101; RRID: AB_10949073 |
| InVivoPlus anti-mouse LAG-3 Antibody, Clone C9B7W |
BioXCell |
Cat# BP0174; RRID: AB_10949602 |
| Chemicals, Peptides, and Recombinant Proteins |
| CFSE |
Thermo Fisher Scientific |
65–0850-84 |
| Cell Stimulation Cocktail (500X) |
Thermo Fisher Scientific |
00–4975-03 |
| H-2Kb:OVA257–64 MHC Class I Monomer |
NIH Tetramer Core |
N/A |
| Streptavidin, R-Phycoerythrin Conjugate (SAPE) |
Thermo Fisher Scientific |
S866 |
| Critical Commercial Assays |
| Fixation/Permeabilization Solution Kit |
BD Biosciences |
554714 |
| Anti-PE Microbeads |
Miltenyi |
Cat# 130–048-801; RRID: AB_244373 |
| Experimental Models: Organisms/Strains |
| BALB/c-Act-2W1S/OVA |
Laboratory of Marc Jenkins |
Published in Moon et al., 2011; Backcrossed 10 generations to BALB/c background |
| C57BL/6NCr |
Charles River Laboratories (NCI) |
#556 |
| Balb/cAnNCr |
Charles River Laboratories (NCI) |
#555 |
| Software and Algorithms |
| FlowJo |
BD Biosciences |
https://www.flowjo.com |
| GraphPad Prism |
GraphPad Software |
https://www.graphpad.com |
