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. Author manuscript; available in PMC: 2020 Jul 27.
Published in final edited form as: Cell Rep. 2020 Jun 23;31(12):107784. doi: 10.1016/j.celrep.2020.107784

KEY RESOURCES TABLE.

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
PE/Cy5 anti-mouse/human CD11b Antibody, Clone M1/70 BioLegend Cat# 101210; RRID: AB_312793
PE/Cy5 anti-mouse/human CD45R/B220 Antibody, Clone RA3-B62 BioLegend Cat# 103210; RRID: AB_312995
PE/Cy7 anti-mouse CD62L Antibody, Clone MEL-14 BioLegend Cat# 104418; RRID: AB_313103
Brilliant Violet 605 anti-mouse Antibody, Clone 29F.1A12 BioLegend Cat# 135220; RRID: AB_2562616
APC anti-mouse CD152 (CTLA-4) Antibody, Clone UC10–4B9 BioLegend Cat# 106310; RRID: AB_2087653
Alexa Fluor 700 anti-mouse/human CD44 Antibody, Clone IM7 BioLegend Cat# 103026; RRID: AB_493713
Brilliant Violet 650 anti-mouse IFN-gamma Antibody, Clone XMG1.2 BioLegend Cat# 505832; RRID: AB_2734492
APC anti-mouse TNF-alpha Antibody, Clone MP6-XT22 BioLegend Cat# 506308; RRID: AB_315429
Brilliant Violet 421 anti-mouse IL-2 Antibody, Clone JES6–5H4 Biolegend Cat# 503826; RRID: AB_2650897
PE/Cy7 anti-mouse CD45.1 Antibody, Clone A20 BioLegend Cat# 110730; RRID: AB_1134168
Brilliant Violet 421 anti-mouse CD45.2 Antibody, Clone 104 BioLegend Cat# 109832; RRID: AB_2565511
eFluor 450 anti-mouse CD4 Antibody, Clone GK1.5 Thermo Fisher Scientific Cat# 48–0041-82; RRID: AB_10718983
APC-eFluor 780 anti-mouse CD8alpha Antibody, Clone 53–6.7 Thermo Fisher Scientific Cat# 7–0081-82; RRID: AB_1272185
PE/Cy5 anti-mouse CD11c Antibody, Clone N418 Thermo Fisher Scientific Cat# 15–0114-82; RRID: AB_468717
PE/Cy5 anti-mouse F4/80 Antibody, Clone BM8 Thermo Fisher Scientific Cat# 15–4801-82; RRID: AB_468798
PE/Cy7 anti-mouse CD197 (CCR7) Antibody, Clone 4B12 Thermo Fisher Scientific Cat# 25–1971-82; RRID: AB_469652
FITC anti-mouse CD223 (LAG-3) Antibody, Clone C9B7W Thermo Fisher Scientific Cat# 11–2231-82; RRID: AB_2572484
eFluor 450 anti-mouse CD366 (TIM3) Antibody, Clone 8B.2C12 Thermo Fisher Scientific Cat# 48–5871-82; RRID: AB_2574081
FITC anti-mouse CD4 Antibody, Clone GK1.5 Thermo Fisher Scientific Cat# 11–0041-85; RRID: AB_464893
PE/Cy7 anti-mouse CD8alpha Antibody, Clone 53–6.7 Thermo Fisher Scientific Cat# 25–0081-82; RRID: AB_469584
PE/Cy7 anti-mouse CD3epsilon Antibody, Clone 145–2C11 Thermo Fisher Scientific Cat# 25–0031-82; RRID: AB_469572
InVivoPlus anti-mouse CD4 Antibody Clone GK1.5 BioXCell Cat# BP0003–1; RRID: AB_1107636
InVivoPlus anti-mouse CD8alpha Antibody, Clone 2.43 BioXCell Cat# BP0061; RRID: AB_1125541
InVivoPlus anti-mouse PD-L1 Antibody, Clone 10F.9G2 BioXCell Cat# BP0101; RRID: AB_10949073
InVivoPlus anti-mouse LAG-3 Antibody, Clone C9B7W BioXCell Cat# BP0174; RRID: AB_10949602
Chemicals, Peptides, and Recombinant Proteins
CFSE Thermo Fisher Scientific 65–0850-84
Cell Stimulation Cocktail (500X) Thermo Fisher Scientific 00–4975-03
H-2Kb:OVA257–64 MHC Class I Monomer NIH Tetramer Core N/A
Streptavidin, R-Phycoerythrin Conjugate (SAPE) Thermo Fisher Scientific S866
Critical Commercial Assays
Fixation/Permeabilization Solution Kit BD Biosciences 554714
Anti-PE Microbeads Miltenyi Cat# 130–048-801; RRID: AB_244373
Experimental Models: Organisms/Strains
BALB/c-Act-2W1S/OVA Laboratory of Marc Jenkins Published in Moon et al., 2011; Backcrossed 10 generations to BALB/c background
C57BL/6NCr Charles River Laboratories (NCI) #556
Balb/cAnNCr Charles River Laboratories (NCI) #555
Software and Algorithms
FlowJo BD Biosciences https://www.flowjo.com
GraphPad Prism GraphPad Software https://www.graphpad.com