Table 1.

Identification of Cinchona‐alkaloid‐inspired inhibitors of starvation and/or rapamycin‐induced autophagy, derived from quinine.

Entry	Compound	R group and position	Starvation‐ Induced IC50 [μm]	Rapamycin‐ Induced IC50 [μm]
1	10 h	5‐CF3	7.86±0.8	n/a
2	10 i	5‐NO2	7.33±1.5	n/a
3	10 k	5‐OCF3	4.68±1.4	n/a
4	10 m	5‐Cl	5.54±2.5	n/a
5	10 n	5‐Br	6.78±1.1	n/a
6	10 r	6‐CF3	5.82±3.0	n/a
7	10 s	6‐Cl	8.12±1.5	n/a
8	10 u	7‐OMe	2.46±0.6	2.37±0.7
9	10 w	7‐azaindole	4.33±1.7	4.95±0.7
10	10 w‐a	4‐Cl‐7‐azaindole	0.52±0.20	0.65±0.35
11	10 w‐b	5‐Me‐7‐azaindole	0.31±0.09	0.86±0.26
12	10 w‐c	5‐Ar‐7‐azaindole	9.00±1.1	n/a
13	10 w‐d	5‐CF3‐7‐azaindole	0.12±0.03	0.77±0.29
14	10 w‐e	5‐NO2‐7‐azaindole	0.67±0.13	1.26±0.20
15	10 w‐f	5‐F‐7‐azaindole	n/a	n/a
16	10 w‐g	5‐Cl‐7‐azaindole	0.11±0.04	0.85±0.14
17	10 w‐h	5‐Br‐7‐azaindole	0.08±0.03	0.81±0.35
18	10 w‐i	5‐I‐7‐azaindole	0.08±0.02	1.24±0.20
19	10 w‐j (azaquindole‐1)	5‐Br‐6‐Me‐7‐azaindole	0.04±0.02	0.10±0.02
20	10 w‐k	6‐Me‐7‐azaindole	3.12±0.5	5.11±1.4
21	10 w‐l	6‐Cl‐7‐azaindole	3.06±0.9	6.21±2.2
22	9 w	7‐azaindole	n/a	nd
23	9 w‐b	5‐Me‐7‐azaindole	n/a	nd

All data are shown as mean ± SD of three independent experiments (N=3; n≥3). All compounds were initially assayed at a concentration of 10 μm. For hits reducing the number of LC3 puncta by more than 50 %, IC₅₀ values were determined. n/a=inactive (no reduction of LC3 puncta at 10 μm). Ar=4‐Cl‐C₆H₄. nd=not determined.