Figure 1.

(a) Extraction of candidate enhancers using evolutionarily conserved noncoding sequences; plot using the comparative genomics alignment tool VISTA; comparison of human (Homo sapiens), mouse (Mus musculus), and frog (Xenopus tropicalis) Pax2 loci; the pink peak indicates the conserved noncoding sequences and the blue peak indicates exons. (b) Extraction of candidate enhancers based on epigenetic profiling of H3K4me1, H3K4me3, and H3K27ac, and the binding of histone acetyltransferase p300; H3K4me1, H3K27ac, and p300 are often associated with enhancers, whereas H3K4me3 and H3K27ac are often present at active promoters. The image was adapted and modified from Prescott et al. (2015)