Figure 3.

(a) Extraction of candidate enhancers for Lhx1 using evolutionarily conserved noncoding sequences; the magenta box indicates the noncoding evolutionarily conserved sequence (CNS) between frogs and fish. The blue box indicates the CNS among vertebrates. (b) Identification of regeneration‐related enhancers for frog nephric tubules; nonmosaic founder frogs are generated by injecting reporter DNA and sperm nuclei into unfertilized eggs. Functional enhancers in regenerating tissues can be identified using founder transgenic animals. (c) Mechanisms of activation of regeneration signal‐response enhancers (RSREs); with the H3K9me3 demethylase–Kdm4 complex, Arid3a binds to RSREs and reduces H3K9me3 levels, thereby promoting the expression of Lhx1 during regeneration of nephric tubules