Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 27;97(6):610–619. doi: 10.1002/cyto.a.24036

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

PMC Copyright notice

Density plots showing measurements of PKH67‐stained M4T1 derived EV in a comparison between the standard configuration of the FSC detector optics (5‐mm obscuration bar, 700‐μm pinhole, A, C) and the optimized configuration (8‐mm obscuration bar, 200‐μm pinhole, B, D, E). Panels A and B are in fluorescence thresholding mode. Panels C–E are in FSC thresholding mode. F Histogram displaying FITC‐MESF calibrated axis for PKH67 stained M4T1 EV, same as shown in Panel B. (G) Bar graph displaying concentration measured with the optimized configuration in fluorescence thresholding mode (threshold value of 0.62) and FSC thresholding mode (threshold values of 0.72 and 0.27) as indicated. Concentration was corrected for dilution, flow rate, time measurement and the number of events detected in PBS background controls (which total numbers of events were 185, 1,456, and 1,522 respectively). [Color figure can be viewed at wileyonlinelibrary.com]