Table 3.
Linearity and efficiency assessment for the pat/bar duplex real time PCR assay
| bar | pat | |||||
|---|---|---|---|---|---|---|
| Slope | R2 | Efficiency (%) | Slope | R2 | Efficiency (%) | |
| Run 1 | −3.24 | 1.000 | 103.4 | −3.42 | 0.999 | 96.1 |
| Run 2 | −3.36 | 0.999 | 98.5 | −3.39 | 1.000 | 97.4 |
| Run 3 | −3.43 | 0.997 | 95.8 | −3.41 | 1.000 | 96.3 |
| Run 4 | −3.34 | 1.000 | 99.4 | −3.44 | 1.000 | 95.2 |
| Average | −3.34 | 0.999 | 99.3 | −3.42 | 1.000 | 96.3 |