Table 1.

Sequences of different HRs in the Cat^hyb.

HR domains	Sequence	Length [nt]	GC content	T m [°C]
G5T5	a1: 5′‐AAAAAGCGCC‐3′ b1: 3′‐TTTTTCGCGG‐5′	10	50.0 %	37.00±0.04
G7T2	a1: 5′‐CGCAGAGCC‐3′ b1: 3′‐GCGTCTCGG‐5′	9	77.8 %	40.50±0.02
G7T3	a1: 5′‐CCTGCTGCTC‐3′ b1: 3′‐GGACGACGAG‐5′	10	70.0 %	47.50±0.02
G9	a1: 5′‐CGCCGGGCC‐3′ b1: 3′‐GCGGCCCGG‐3′	9	100.0 %	55.00±0.02