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Primer extension with a KOD XL polymerase using either (A) 19‐mer template tempA or tempC and primrnd; or (B) 31‐mer temprnd16 and primrnd: (P) primer (5′‐6‐FAM‐labelled); (A+, C+ or +) natural dNTPs; (A‐) dCTP, dTTP, dGTP; (C‐) dATP, dTTP, dGTP; (AFc) dAFcTP, dCTP, dTTP, dGTP; (CFc) dCFcTP, dATP, dTTP, dGTP; (AFcMe) dAFcMeTP, dCTP, dTTP, dGTP; (CFcMe) dCFcMeTP, dATP, dTTP, dGTP; (AFcPa) dAFcPaTP, dCTP, dTTP, dGTP; (CFcPa) dC FcPa TP, dATP, dTTP, dGTP.
