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. 2020 Feb 20;129(2):378–388. doi: 10.1111/jam.14608

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© 2020 The Authors. Journal of Applied Microbiology published by John Wiley & Sons Ltd on behalf of Society for Applied Microbiology

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Escherichia coli, Bifidobacterium spp. and total bacterial gene copies per gram faeces. (a, c and e) the five DNA extraction assays, and (b, d and f) the three different pre‐treatment methods for the PowerMicrobiome assay. Data presented as median with 95% CI; EM: Semi‐automated NucliSens easyMag; QIA: QIAamp DNA Stool mini kit; PL: PureLink Microbiome DNA Purification kit; PF: QIAamp PowerFecal DNA kit; PM: RNeasy PowerMicrobiome kit; PK: Proteinase K pre‐treatment; M: mutanolysin pre‐treatment; B: bead beating pre‐treatment; *P < 0·050; **P ≤ 0·001.