. 2020 Feb 20;129(2):378–388. doi: 10.1111/jam.14608

Table 1.

qPCR primers and probe sequences

Target species (gene)	Sequence 5′–3′	Amplicon length (bp)	Reference
Bifidobacterium spp. (16S rRNA gene)	F: GAATAGCTCCTGGAAACG R: ATAGGACGCGACCCCA	99	This paper This paper
Escherichia coli (uidA gene)	F: CAACGAACTGAACTGGCAGA R: CATTACGCTGCGATGGAT P: FAM‐TATCCCGCCGGGAATGGTGA‐TAMRA	121	Chern et al. (2011) Chern et al. (2011) This paper
Total bacteria (16S rRNA gene)	F: CTCCTACGGGAGGCAGCAGT R: GTATTACCGCGGCTGCTG	170–200	Vaneechoutte et al. (2000) Vaneechoutte et al. (2000)

Target species (gene)

Sequence 5′–3′

Amplicon length (bp)

Reference

Bifidobacterium spp. (16S rRNA gene)

F: GAATAGCTCCTGGAAACG

R: ATAGGACGCGACCCCA

This paper

Escherichia coli (uidA gene)

F: CAACGAACTGAACTGGCAGA

R: CATTACGCTGCGATGGAT

P: FAM‐TATCCCGCCGGGAATGGTGA‐TAMRA

121

Chern et al. (2011)

This paper

Total bacteria (16S rRNA gene)

F: CTCCTACGGGAGGCAGCAGT

R: GTATTACCGCGGCTGCTG

170–200

Vaneechoutte et al. (2000)

F: forward primer; R: reverse primer; P: probe; bp: base pair; FAM: 6‐carboxyfluorescein, fluorescence reporter dye; TAMRA: 5(6)‐carboxy‐tetramethylrhodamine, fluorescence quencher dye.