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. 2020 May 3;103(1):197–211. doi: 10.1111/tpj.14719

Figure 4.

Figure 4

Statistical analysis of the transcript profiles of various soybean organs after exposure to dehydration. (a) Correlation analysis of gene transcript levels. Values on the y and x axes indicate fold change in microarray and CAGE analyses, respectively. (b) Principal component analysis (PCA) of gene transcript levels. Values on the y and x axes indicate PC1 and PC2, respectively. (c) PCA loadings for gene transcripts. (d) Expression levels of representative genes under dehydration conditions. In each case, the maximum gene transcript level was set to 100. Values are the mean ± SD (n = 3 experiments). (e) Molecular functions of dehydration‐responsive genes in various soybean organs. Radar charts of 27 upregulated and downregulated molecular function classes indicating the frequency of each class in dehydration‐treated soybean organs. 1, Carbohydrate metabolism; 2, Energy metabolism; 3, Lipid metabolism; 4, Nucleotide metabolism; 5, Amino acid metabolism; 6, Metabolism of other amino acids; 7, Glycan biosynthesis and metabolism; 8, Metabolism of cofactors and vitamins; 9, Metabolism of terpenoids and polyketides; 10, Biosynthesis of other secondary metabolites; 11, Xenobiotic biodegradation and metabolism; 12, Biosynthesis of plant hormones; 13, Photosynthesis; 14, Signal transduction; 15, Transcription; 16, Translation; 17, Epigenetics; 18, Molecular chaperone; 19, DNA replication and repair; 20, Helicase; 21, Ribonuclease; 22, Ubiquitination; 23, Protease/peptidase; 24, Transport; 25, Detoxification enzyme; 26, dehydrin/LEA proteins; 27, Other. Orange and light blue indicate the 3‐day dehydration treatment. Red and blue indicate the 4‐day dehydration treatment. (F, G) Heatmaps illustrate the log ratios of dehydration‐responsive genes in representative molecular classes of dehydrin/LEA proteins (f) and photosynthesis (g). psaD, photosystem I subunit II; psbO, photosystem II oxygen‐evolving enhancer protein 1; Lhc, chlorophyll a/b‐binding protein; atpC, CF1‐ATPase γ‐subunit; rbcS, ribulose 1,5‐bisphosphate carboxylase/oxygenase small subunit.