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. Author manuscript; available in PMC: 2020 Dec 8.
Published in final edited form as: Nat Metab. 2020 Jun 8;2(7):620–634. doi: 10.1038/s42255-020-0217-6

Fig. 1: Stat3 is phosphorylated in response to β-adrenergic receptor activation in adipocytes.

Fig. 1:

a. Il6 expression in 12-week high fat diet (HFD) fed C57BL/6 mice treated with 1 mg/kg CL-316,243 or vehicle control for 30 minutes before sacrifice and tissue collection. Expression levels normalized to vehicle within each tissue. Individual data points plotted ± SEM (n = 12 mice per treatment group). p value = 0.0003 (iWAT) and 0.006 (eWAT) b. Serum IL-6 levels in 12-week HFD fed C57BL/6 mice treated with 1 mg/kg CL-316,243 or vehicle control before sacrifice and blood collection. Data are represented as mean ± SEM (n = 3 mice per genotype at each time point). p value < 0.0001 WT vs Il6 KO. Quantification of phosphorylation of c. HSL at serine 563 over total HSL (p value < 0.0001 20 min vs. basal) d. p38 at threonine 180 and tyrosine 182 over total p38 (p value = 0.003 20 min vs. basal) e. STAT3 at tyrosine 705 over total STAT3 (p value < 0.0001 120 min vs. basal) and f. STAT3 at serine 727 over total STAT3 (p value = 0.003 20 min vs. basal). c.-f. Western blots shown in Extended Data Fig. 1. Individual data points plotted ± SEM (n = 3 WT, 4 SAKO mice per time point). * p value < 0.05 from post hoc analysis after significant two-way ANOVA.