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. Author manuscript; available in PMC: 2020 Jul 27.
Published in final edited form as: Am J Clin Pathol. 2011 Jun;135(6):890–900. doi: 10.1309/AJCPV2D1DDSGJDBW

▮Image 1▮.

▮Image 1▮

Effect of gating strategy on Vβ analysis. A, Initial lymphocyte gate (blue) was established based on FSC and SSC properties. Subsequently, 2 gating strategies were used. B, Gating strategy 1 gates all CD3+/CD4+ cells (red) to examine the Vβ distribution. C, Vβ17 expression is noted in 18.25% of CD3+/CD4+ cells. D, Gating strategy 2 gates specifically on neoplastic cells of interest, by gating on dim CD3+ and CD4+ cells (red). E, Vβ17 expression is noted in 72.48% of dim CD3+ and CD4+ cells. APC, allophycocyanin; FITC, fluorescein isothiocyanate; FSC, forward light scatter; PE, phycoerythrin; PerCP, peridinin chlorophyll-a protein; SSC, side light scatter.