Fig. 10.

PAR4 mediates thrombin-stimulated IL-1β secretion from human cardiac fibroblasts. a PAR1, PAR2 and PAR4 protein expression, normalized to γ-tubulin, in whole cell lysates of human ventricular fibroblasts maintained under low glucose (LG, 5.5 mmol/L) or high glucose (HG, 25 mmol/L) conditions (n = 3 individual experiments). b Il1b mRNA expression and c IL-1β secretion determined in HG-fibroblast cultures stimulated with thrombin (Thr, 3 U/mL) ± ML354 (PAR4 inhibitor, 200 nmol/L) for 24 h (n = 3 individual experiments). Data show mean ± SD, relative to control (Ctl). *P < 0.05 vs. corresponding LG, Ctl or Thr