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. 2020 Jul 27;1864(11):129694. doi: 10.1016/j.bbagen.2020.129694

Fig. 3.

Fig. 3

Cellular phenotype of COG KO cells. Upper panel: (A) EM of HeLa COG3 KO cells shows a fragmented Golgi with dilated cisternae, unlike the well-organized stacks in the WT. (B) Superresolution microscopy of HEK293T WT and COG4 KO cells transfected with ST6Gal1-RFP and GFP-STX5 (WT; Golgi marker) or Lamp2-GFP (COG4 KO; Endolysosomal marker). Golgi enzyme ST6Gal1 is mislocalized to Lamp2 labeled endolysosomes and EELSs. Asterisks indicate the Golgi. Arrows point to EELSs, which are 1-10 μM in diameter and accumulate in COG KO cells.