Figure 8. Preincubation of PVN slices in 200 nM oxytocin produces an Oxtr dependent increase in holding current and current density.
A) Slices incubated 200 nM oxytocin for a minimum of one hour prior to whole-cell recording were compared to control slices from the same animals incubated in normal ACSF. Results indicated that preincubation in 200 nM oxytocin produced a clear increase in holding current and current density relative to preparation matched controls. Asterisks indicated P<0.05, see text in the results section for further details. B) The effects produced by preincubation of slices in 200 nM oxytocin presented in panel A were not observed in slices that were incubated in 200 nM oxytocin + 1 μM L-368. This result highlights a clear role for Oxtr(s) in producing the inhibitory effect of preincubation highlighted in panel A. See text of the results for further details.